Oxoid LAURYL TRYPTOSE BROTH CM0451B

Oxoid Lauryl Tryptose Broth CM0451B

Oxoid Lauryl Tryptose Broth CM0451B is a selective medium formulated for the detection of coliform organisms in water, dairy products, and other foodstuffs. It is commonly used in the presumptive phase of the Most Probable Number (MPN) technique to identify coliforms through lactose fermentation with gas production. This medium contains tryptose and lactose to support bacterial growth, while sodium lauryl sulfate serves as a selective agent to inhibit non-coliform organisms.

Features

• selective for coliforms – contains sodium lauryl sulfate to inhibit non-coliform bacteria

• supports gas production – reliable indicator of lactose-fermenting coliform activity

• high-quality formulation – consistent and reproducible results in microbiological testing

• compatible with MPN method – standard medium for presumptive coliform detection

• easy preparation – dissolves quickly and is ready for sterilization

• meets standard methods – complies with international guidelines for water and food microbiology

Application

• water testing – used in potable, recreational, and wastewater analysis for coliform presence

• dairy microbiology – monitoring of coliforms in milk, cheese, and other dairy products

• food safety – applied in the examination of raw and processed foods for hygiene indicators

• beverage industry – routine testing of juices, bottled drinks, and brewing materials

• pharmaceutical testing – employed in microbial limits testing of non-sterile products

• educational and research laboratories – a standard for training and microbiological studies

Specification

• product name: Oxoid Lauryl Tryptose Broth CM0451B

• format: dehydrated culture medium

• typical composition per liter:

  • tryptose: 20.0 g

  • lactose: 5.0 g

  • sodium chloride: 5.0 g

  • sodium lauryl sulfate: 0.1 g

• final pH: 6.8 ± 0.2 at 25 °C

• appearance: free-flowing, light beige powder

• preparation: dissolve 30.1 g in 1 liter of distilled water, distribute into tubes with inverted Durham tubes, sterilize at 121 °C for 15 minutes

• storage conditions: store tightly closed in a dry place at 10 – 30 °C, protect from light and moisture

• shelf-life: use before expiry date indicated on packaging

Conclusion

Oxoid Lauryl Tryptose Broth CM0451B is a trusted and standardized medium for the selective detection of coliforms in various sample types. Its reliable performance in gas detection and ease of preparation make it an essential tool in routine microbiological quality control and regulatory compliance across industries.